L.I. Medved’s Research Center of Preventive Toxicology, Food and Chemical Safety, Ministry of Health, Ukraine (State Enterprise), Kyiv, Ukraine
Abstract. Nitrates are natural metabolic products of all plants. They are vital to plants – without them their normal growth and development is impossible. However, the uncontrolled use of nitrogen fertilizers has led to their uncontrolled accumulation in food products. When eating vegetables with high amounts of nitrates, they are partially restored to nitrites in the intestinal tract, and the latter, entering the bloodstream cause diseases – methemoglobinemia. Therefore, quantitative and qualitative determination of nitrates and nitrites in plant and animal products, water and soils are relevant today. Based on the analysis of scientific literature, the essence of the problem of excessive accumulation of nitrates in agricultural products and their negative impact on human health is highlighted.
Aim. The aim of the research was to determine experimentally the concentration of nitrates and nitrites in plant and animal products, water and soils, to compare it with the maximum allowable concentration as required by Commission Regulation (EU) No 1258/2011 on maximum nitrate content in vegetables and by State sanitary rules and regulations on Maximum Permissible Levels of Certain Contaminants in Food, approved by the Order of the Ministry of Health of Ukraine of May 13, 2013 No 368 (as amended by the order of the Ministry of Health of Ukraine of May 22, 2020 No 1238).
Materials and Methods. Sources of information retrieval in printed and electronic publications, databases search engines, methods of analysis, comparison and generalization of data. Photometric method.
Results. The analysis of the value of nitrates in nature, sources of this substance in food was conducted; normalization of the content of nitrate components, the problem of plant products quality decline due to the uncontrolled use of fertilizers was studied; the content of nitrates in plant and animal products; the harmful effects of products with high nitrate content were considered; a comprehensive assessment of nitrate content in plant products was carried out; comparative characteristics of methods for determination of nitrate compounds were given; the consequences of consumption of products with high nitrate content were analyzed.
Conclusions. The problem of nitrate pollution is becoming increasingly significant, because the amount and speed of the nitrate nitrogen cycle in the environment is constantly increasing, which increases its impact on natural systems and the possibility of nitrate accumulation in crop products, humans and animals. Therefore, the implementation of analytical control of food and environmental objects is important to obtain objective information about the content of man-made pollutants, which are nitrates and nitrites.
Key Words: nitrates, maximum permissible level, nitrate compounds, nitrate pollution.
СПИСОК ВИКОРИСТАНИХ ДЖЕРЕЛ / REFERENCES
1. Державні санітарні правила і норми «Максимально допустимі рівні окремих забруднюючих речовин у харчових продуктах», затвердженими Наказом Міністерства охорони здоров’я України 13 травня 2013 року № 368 (у редакції наказу Міністерства охорони здоров’я України від 22 травня 2020 року № 1238), зареєстровані в Міністерстві юстиції України 21 липня 2020 р. за № 684/34967
2. Дерягина ВП. Разработка методов анализа нитратов и нитритов в пищевых продуктах и гигиеническая оценка способов снижения их содержания при промышленной и кулинарной переработке // [Автореферат] Москва; 1994. 24 с.
3. ДСТУ 4078-2001 «Якість води. Визначання нітрату. Частина 3. Спектрометричний метод із застосовуванням сульфосаліцилової кислоти (ISO 7890-3:1988, MOD)»
4. ДСТУ ISO 7890-1:2003 «Якість води. Визначання нітрату. Частина 1. Спектрометричний метод із застосовуванням 2,6- диметилфенолу (ISO 7890-1:1986, IDT)»
5. Ильницкий АП. Нитраты и нитриты питьевой воды как фактор онкологического риска. Гигиена и санитария. 2003; 6: 81-4.
6. Кузубова ЛИ. Токсиканты в пищевых продуктах. Новосибирск; 1990. 126 с.
7. Митченков ВТ. Токсиколого-гигиеническая оценка нитратно-нитритной нагрузки на организм человека и методические основы ее профилактики [Автореферат] Москва; 1992. 41 с.
8. Мурох ВИ. Гигиеническая оценка пищевых продуктов в условиях интенсивно применения минеральных удобрений в Белорусской ССР [Автореферат] Москва; 1989. 49 с.
9. Ополь НИ. Добрянская ЕВ. Нитраты. Кишинев: «Штиинца»; 1986. 114 с.
10. Панасенко ЕІ, Красноруцька КІ. Вміст нітратіонів в продуктах харчування рослинного походження. Актуальні питання біології, екології та хімії. Розділ хімія. 2016; том.12, (2): 103-12.
11. Панчеко ТІ., Мандебура СВ. Оцінка вмісту нітратів в продуктах рослинного походження.
12. URL:htpp://ir.lib/vntu.edu.ua/bitstream/handle/123456789/2672912716/pdf?sequence=Allowed=I.
13. Половец ЯВ. Причины накопления и способы уменьшения избыточного количества нитратов в культурных растениях. Молодой ученый. 2019; 23 (261): 154- 7.
14. Танчук ВД, Христіансен ОМ, Бутенко ОМ, Біла ГМ, Дроков ВГ. Моніторинг нітратів та заходи щодо їх зменшення у рослинній продукції. Восточно-Европейский журнал передовых технологий. ISSN 1729-3774. 2012; 6/6(60): 47-8.
15. Цыганенко ОИ. Нитраты в пищевых продуктах. Киев: «Здоров’я»; 1990.
16. Харитонов ММ, Лазарєва ОМ. Екологічна оцінка варіабельності вмісту нітратів у овочевих та плодово-ягідних культурах у Дніпропетровській області. Вісник Полтавської державної аграрної академії. 2015; 3: 29-31.
17. COMMISSION REGULATION (EU) No 1258|2011 of 2 December 2011 amending Regulation (EC) No 1881/2006 as regards maximum levels for nitrates in foodstuffs;
18. COMMISSION REGULATION (EU) No 1881|2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs
19. EC (European Commission). 1997. Opinion on nitrate and nitrite. Reports of the Scientific Committee for Food (SCF) 38 Series, 1-33. Available at URL: http://ec.europa.eu/food/fs/sc/scf/reports/scf_reports_38.pdf
20. EC (European Commission). 1992. Opinion on nitrate and nitrite. Reports of the Scientific Series, 21-28. Available at URL: Committee for Food (SCF) 26 http://ec.europa.eu/food/fs/sc/scf/reports/scf_reports_26.pdf.
21. EFSA. EFSA Panel on Contaminants in the Food Cham (CONTAM): Scientific Opinion. Nitrate in vegetable EFSA J 2008, 689:1-79. Question No EFSA-Q-2006-071, adopted on 10 April 2008.
22. EC Europen Commission Directorate-general III Industry, Scientific Committee for Food. Opinion on nitrate and nitrite expressed on 22 September 1995. Annex 4 to Documeyt 111/5611/95;
23. Evaluation of certain food additives and contaminants. Forty-fourth report of the Joint FAO/WHO Expert Committee on Food Additives» // WHO Technical Report Series 859. Geneva; 1995: 29-35.
24. FAO/WHO (Food and Agriculture Organisation of the United Nations/World Health Organization). 2003. Nitrate (and potential endogenous formation of N-nitroso compounds). WHO Food Additive series 50, Geneva: World Health Organisation. Available at URL: http://www.inchem.org/documents/jecfa/jecmono/v50je06.htm.
25. FAO/WHO (Food and Agriculture Organisation of the United Nations/World Health Organization). 2003. Nitrite (and potential endogenous formation of N-nitroso compounds). WHO Food Additive series 50, Geneva: World Health Organisation. Available
26. Lundberg JO, Weitzberg E, Cole JA. and Benjamin N. Nitrate, bacteria and human health. Nat.Rev.Microbiol. 2004; 2: 593-602.
27. Lundberg JO, Weitzberg E and Gladwin MT. The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nature Reviews Drug Discovery. 2008; 7: 156 - 67.
28. Mensinga TT, Speijers GJA and Meulenbelt J. Health implications of exposure to environmental nitrogenous compounds. Toxicological Reviews. 2003; 22: 41-51. HUdoi:10.2165/00139709-200322010-00005U
29. Nitrate in vegetables. Scientific Opinion of the Panel on Contaminants in the Food chain. The EFSA Journal. 2008; 689: 1-79.
30. Opinion of Scietific Panel on Contaminants in the Food chain on reguest from the European Commission – to perform a scientific risk assessment on nitrate in vegetables, EFSA Journal . 2008; 689: 1;
31. IPCS; Poisons Information Monograph G016: Nitrates and nitrites. (September 1996). Available from, as of October 24, 2006: http://www.inchem.org/documents/pims/chemical/pimg016.html.
32. Santamaria P. Nitrate in vegetables: toxicity content, intake and EC regulation J.Food Agric. 2006; 86: 10-17.
Стаття надійшла до редакції 22.11.2021 р. / Received November 22, 2021.