N. Filipets1, L. Vlasyk1,4, O. Gerush1, O. Filipets1, O. Kmet1, L. Gabunia2, A. Gozhenko3
1Bukovinian State Medical University, Chernivtsi, Ukraine
2Tbilisi State Medical University, Tbilisi, Georgia
3Ukrainian Research Institute of Transport Medicine, Odesa, Ukraine
4L.I. Medved’s Research Centre of Preventive Toxicology, Food and Chemical Safety, Ministry of Health, Ukraine (State Enterprise), Kyiv, Ukraine
Abstract. When conducting experimental studies, the question of an adequate model of renal pathology is problematic, therefore, the range of choice of methods of reproduction is extremely wide. The paper highlights the main modern methods of modeling and the principles of choosing a model of nephropathy for the study of changes in kidney functions and processes. It also investigates the effectiveness of pharmacological correction under the conditions of exposure to exotoxins. Attention is focused on models with predominant damage to nephron sections with established pathogenetic features of the course of acute and chronic toxic nephropathy.
Aim of research is elucidation of methods of modeling toxic nephropathies, using the results of our research laboratories and modern data from the literature on experimental nephrology.
Materials and Methods. Analytical methods are used in the work: collection of scientific information on the problem, data analysis and scientific generalization of results.
Results and Conclusions. Experimental models of kidney diseases are diverse, which allows to carefully study the pathogenesis of renal pathology and to develop effective therapeutic strategies. At the same time, in experimental studies, in a number of cases, it is necessary to simulate kidney disease with differentiated damage to the glomerular or tubular part of the nephron. The development of effective medical interventions to reduce exotoxin-induced nephrotoxicity largely depends on the chosen experimental model, respectively, on the understanding of the pathophysiology of toxic nephropathies.
Keywords: experimental nephrology, differentiated damage of the nephron, toxic nephropathies.
СПИСОК ВИКОРИСТАНИХ ДЖЕРЕЛ / REFERENCES
1. Gao Z, Wu N, Du X, Li H, Mei X, Song Y. Toxic Nephropathy Secondary to Chronic Mercury Poisoning: Clinical Characteristics and Outcomes. Kidney Int Rep. 2022 Mar 18;7(6):1189-1197.doi: 10.1016/j.ekir.2022.03.009.
2. Іvanov DD. Toxic nephropathy. Medytsyna neotlozhnыkh sostoianyi. 2014;(2):164-165.
3. Friederich-Persson M, Thörn E, Hansell P, NangakuM, Levin M, Palm F. Kidney hypoxia, attributable to increased oxygen consumption, induces nephropathy independently of hyperglycemia and oxidative stress. Hypertension. 2013 Nov;62(5):914-9. doi: 10.1161/HYPERTENSIONAHA.113.01425. Epub 2013 Sep 9.
4. Pishak VP, Gozhenko AI, Rogovy YuE. Tubulo-interstitial syndrome. Chernivtsi – Odesa: Medical Academy; 2002. 221 с.
5. Gozhenko AI. Pathogenesis of toxic nephropathies. Actual problems of transport medicine. 2006;(2):9-15.
6. Boychuk TM, Gozhenko AI, Filipets ND. Khodorovskyy VM. The pathogenetic mechanisms of acute kidney injury and chronic kidney disease. Zaporozhye Medical Journal. 2018;20(2):259-264.
7. Gozhenko AI. The nephrotoxic effect of sublimate on rats depending on sodium intake. Physiology and pathology of the cardiovascular system and kidneys. Cheboksary: Chuvash State University; 1982. p. 126-128.
8. Fontana F, Cazzato S, Giovanella S, Ballestri M, Leonelli M, Mori G, et al. Oxalate Nephropathy Caused by Excessive Vitamin C Administration in 2 Patients With COVID-19. Kidney Int Rep. 2020 Oct;5(10):1815-1822. doi: 10.1016/j.ekir.2020.07.008. Epub 2020 Jul 16.
9. Karchauskas VYu. Pathophysiology of kidneys and osmotic homeostasis under conditions of cadmium nephropathy [dissertation abstract]. Odesa: Odessa State Medical University; 2009. 18 с.
10. Boychuk TM, Kmet TI, Filipets ND, Vlasyk LI, Hrachova TI. Functional renal state of mature rats with a quick type of acetylation under conditions of cadmium chloride and sodium nitrate. Fiziolohichnyi zhurnal. 2018;64(6):3-8.
11.Filipets ND, Kmet TI, Hrachova TI. Peculiarities of water-electrolytic metabolism of rats with «slow» type of acetylation after a combined effect of cadmium chloride and sodium nitrate. Fiziolohichnyi zhurnal. 2018;64(4):51-56.
12. Gozhenko AI, Dolomatov SI, Babyin IYu, Nasibullin BА. Renal mechanisms of regulation of the nitrogen oxide cycle in white rats loaded with sodium nitrite. Nephrology. 2005;9(3):95-98.
13. Gozhenko AI, Dolomatov SI, Dolomatova ЕА. Reactions of white rats kidneys to small doses of sodium nitrite. Nephrology. 2004;8(2):86-89.
14. Gozhenko AI, Fedoruk АS, Коtiuzhinskaya SG, Gozhenko AI, Кuzmenko IА. Changes in kidney function in acute intoxication with sodium nitrite in the experiment. Pathological physiology and experimentaltherapy. 2003;(1):28-30.
15. Gozhenko AI, Filipets ND. The functional state of kidneys after adenosine triphosphatesensitive potassium channels activation in experimental acute hypoxia.Fiziolohichnyi zhurnal. 2014;60(4):22-29.
16. Adewale OO, Bakare MI, Adetunji JB. Mechanism underlying nephroprotective property of curcumin against sodium nitrite-induced nephrotoxicity in male Wistar rat. J Food Biochem. 2021;45(3):e13341. doi: 10.1111/jfbc.13341.
17. Rohovyy Y, Doroshko V. Early pathogenesis mechanisms of pseudohepatorenal syndrome as the basis to deteriorate the course of kidney and liver failure under conditions of 2,4-dinitrofenol administration. International Scientific Journal. 2015;(4):23-27.
18. Gozhenko AI, Moskalenko AM, Sirman VM, Zhizhnevskaya АА, Storozhenko SA. Kidney function in oncologic patients after cisplastin chemotherapy. Fiziolohichnyi zhurnal. 2012;58(3):73-76.
19. Ozkok A, Edelstein CL. Pathophysiology of cisplatininduced acute kidney injury. Biomed Res Int. 2014;2014:967826. doi: 10.1155/2014/967826. Epub 2014 Aug 6.
20. Perše M, Večerić-Haler Ž. Cisplatin-Induced Rodent Model of Kidney Injury: Characteristics and Challenges. Biomed Res Int. 2018 Sep 12;2018:1462802. doi: 10.1155/2018/1462802.
Стаття надійшла до редакції 20.06.2023 р.
Received June, 20, 2023.